{"id":3515,"date":"2021-07-20T10:16:55","date_gmt":"2021-07-20T01:16:55","guid":{"rendered":"http:\/\/hyperresearch.com\/wp\/?p=3515"},"modified":"2022-05-01T16:50:29","modified_gmt":"2022-05-01T07:50:29","slug":"post-3515","status":"publish","type":"post","link":"http:\/\/hyperresearch.com\/wp\/2021\/07\/20\/bio-data-processing\/post-3515\/","title":{"rendered":"\u30a2\u30c0\u30d7\u30bf\u30fc\u9664\u53bb\u30bd\u30d5\u30c8Trimmomatic\u306e\u30a4\u30f3\u30b9\u30c8\u30fc\u30eb\u3068\u5b9f\u884c"},"content":{"rendered":"

Trimmomatic\u306f<\/h3>\n

\u30ea\u30fc\u30c9\u30c7\u30fc\u30bf\u304b\u3089\u3001\u30a2\u30c0\u30d7\u30bf\u30fc\u3092\u9664\u53bb\u3057\u3001\u66f4\u306b\u54c1\u8cea\u306e\u60aa\u3044\u3082\u306e\u3092\u9664\u53bb\uff08\u30c8\u30ea\u30df\u30f3\u30b0\uff09\u3059\u308b\u30b9\u30c6\u30c3\u30d7\u3002\u3069\u3046\u3044\u3046\u54c1\u8cea\u3092\u9664\u53bb\u306e\u5bfe\u8c61\u3068\u3059\u308b\u304b\u3001\u5224\u5b9a\u3092\u3069\u3046\u3059\u308b\u304b\u306a\u3069\u3001\u3044\u308d\u3044\u308d\u306a\u8b70\u8ad6\u304c\u3042\u308b\u3088\u3046\u3067\u3042\u308b\u3002<\/p>\n

\n QC | RNA-Seq \u30ea\u30fc\u30c9\u306e\u30af\u30aa\u30ea\u30c6\u30a3\u30c1\u30a7\u30c3\u30af
\n https:\/\/bi.biopapyrus.jp\/rnaseq\/qc\/\n<\/p><\/blockquote>\n

\u30a2\u30c0\u30d7\u30bf\u30fc\u9664\u53bb\u3068\u7c21\u5358\u306a\u30c8\u30ea\u30df\u30f3\u30b0\u3092\u884c\u3046 trimmomatic \u3092\u7d39\u4ecb\u3059\u308b\u3002<\/p>\n

USADELLAB.org – Trimmomatic: A flexible read trimming tool for Illumina NGS data
\nhttp:\/\/www.usadellab.org\/cms\/?page=trimmomatic<\/a><\/p>\n

\"\"<\/p>\n

bioconda\u3067\u306e\u30a4\u30f3\u30b9\u30c8\u30fc\u30eb\u306f\u3001\u4ed6\u306e\u3088\u304f\u4f7f\u308f\u308c\u308b\u30bd\u30d5\u30c8\u3068\u540c\u69d8\u306b\u65e2\u306b\u6e08\u3093\u3067\u3044\u308b\u306f\u305a\u3060\u304c\u3001\u3082\u3057\u8d77\u52d5\u306b\u5931\u6557\u3059\u308b\u3088\u3046\u306a\u3089\u3070\u4e0b\u8a18\u306e\u3088\u3046\u306b\u3000conda install<\/code> \u306b\u3088\u3063\u3066\u30a4\u30f3\u30b9\u30c8\u30fc\u30eb\u3067\u304d\u308b\u3002<\/p>\n

$ conda install -c bioconda trimmomatic\n<\/code><\/pre>\n
\u3068\u3059\u308b\u3002<\/p>\n
Trimmologic\u306f\u8d77\u52d5\u6642\u306b\u3044\u304f\u3064\u304b\u306e\u30d1\u30e9\u30e1\u30fc\u30bf\u3092\u4e0e\u3048\u308b\u3002\u30d1\u30e9\u30e1\u30fc\u30bf\u306b\u306f\u3001\u30b7\u30f3\u30b0\u30eb\u30a8\u30f3\u30c9\u304b\u30da\u30a2\u30a8\u30f3\u30c9\u304b\u306e\u533a\u5225\u3001\u5165\u529b\u30d5\u30a1\u30a4\u30eb\u3001\u51fa\u529b\u30d5\u30a1\u30a4\u30eb\u306e\u6307\u5b9a\u306e\u307b\u304b\u3001\u9664\u53bb\u3059\u308b\u30a2\u30c0\u30d7\u30bf\u30fc\u3092\u66f8\u3044\u305f\u30d5\u30a1\u30a4\u30eb\u306e\u6307\u5b9a\u3001\u30c8\u30ea\u30df\u30f3\u30b0\u306e\u6761\u4ef6\u306e\u30d1\u30e9\u30e1\u30fc\u30bf\u306a\u3069\u3092\u4e0e\u3048\u308b\u3002\u4e0a\u8a18\u30de\u30cb\u30e5\u30a2\u30eb\u30da\u30fc\u30b8\u306e\u30da\u30a2\u30a8\u30f3\u30c9\u306e\u4f8b\u3067\u306f<\/p>\n
$ trimmomatic PE \\\n  input_forward.fq.gz input_reverse.fq.gz \\\n  output_forward_paired.fq.gz \\\n  output_forward_unpaired.fq.gz \\\n  output_reverse_paired.fq.gz \\\n  output_reverse_unpaired.fq.gz \\\n  ILLUMINACLIP:TruSeq3-PE.fa:2:30:10:2:keepBothReads \\\n  LEADING:3 TRAILING:3 MINLEN:36\n<\/code><\/pre>\n
\u306a\u3069\u306e\u3088\u3046\u306b\u6307\u5b9a\u3057\u3066\u3044\u308b\u3002\u30c8\u30ea\u30df\u30f3\u30b0\u30d1\u30e9\u30e1\u30fc\u30bf\u306e\u5024\u306e\u9078\u629e\u306f\u305d\u308c\u305e\u308c\u306e\u30c7\u30fc\u30bf\u3084\u7528\u9014\u306b\u3088\u3063\u3066\u8abf\u6574\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u3002\u307e\u305f\u30a2\u30c0\u30d7\u30bf\u30fc\u306e\u914d\u5217\u306f\u9069\u5b9c\u30e1\u30fc\u30ab\u30fc\u7b49\u304b\u3089\u5165\u624b\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u3002<\/p>\n
\n
FastQC\u306e\u30a4\u30f3\u30b9\u30c8\u30fc\u30eb\u3068\u5b9f\u884c<\/a>\u3000\uff1c\u3000\u30a2\u30c0\u30d7\u30bf\u30fc\u9664\u53bb\u30bd\u30d5\u30c8Trimmomatic\u306e\u30a4\u30f3\u30b9\u30c8\u30fc\u30eb\u3068\u5b9f\u884c\u3000\uff1e\u3000\u307b\u3052\u307b\u3052<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"
Trimmomatic\u306f \u30ea\u30fc\u30c9\u30c7\u30fc\u30bf\u304b\u3089\u3001\u30a2\u30c0\u30d7\u30bf\u30fc\u3092\u9664\u53bb\u3057\u3001\u66f4\u306b\u54c1\u8cea\u306e\u60aa\u3044\u3082\u306e\u3092\u9664\u53bb\uff08\u30c8\u30ea\u30df\u30f3\u30b0\uff09\u3059\u308b\u30b9\u30c6\u30c3\u30d7\u3002\u3069\u3046\u3044\u3046\u54c1\u8cea\u3092\u9664\u53bb\u306e\u5bfe\u8c61\u3068\u3059\u308b\u304b\u3001\u5224\u5b9a\u3092\u3069\u3046\u3059\u308b\u304b\u306a\u3069\u3001\u3044\u308d\u3044\u308d\u306a\u8b70\u8ad6\u304c\u3042\u308b\u3088\u3046\u3067\u3042\u308b\u3002 QC | RN […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[19],"tags":[],"class_list":["post-3515","post","type-post","status-publish","format-standard","hentry","category-bio-data-processing"],"jetpack_featured_media_url":"","_links":{"self":[{"href":"http:\/\/hyperresearch.com\/wp\/wp-json\/wp\/v2\/posts\/3515","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/hyperresearch.com\/wp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/hyperresearch.com\/wp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/hyperresearch.com\/wp\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/hyperresearch.com\/wp\/wp-json\/wp\/v2\/comments?post=3515"}],"version-history":[{"count":6,"href":"http:\/\/hyperresearch.com\/wp\/wp-json\/wp\/v2\/posts\/3515\/revisions"}],"predecessor-version":[{"id":3755,"href":"http:\/\/hyperresearch.com\/wp\/wp-json\/wp\/v2\/posts\/3515\/revisions\/3755"}],"wp:attachment":[{"href":"http:\/\/hyperresearch.com\/wp\/wp-json\/wp\/v2\/media?parent=3515"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/hyperresearch.com\/wp\/wp-json\/wp\/v2\/categories?post=3515"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/hyperresearch.com\/wp\/wp-json\/wp\/v2\/tags?post=3515"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}